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About the Role
We are seeking an Associate Scientist I to develop, optimize and run biochemical binding assays that screen compounds in medium- to high-throughput format. You will own AlphaScreen/AlphaLISA assays from reagent qualification through routine screening, generating the potency and selectivity data that drive our medicinal chemistry decisions. This is a hands-on laboratory role reporting to Senior Vice President, Research based in South San Francisco.
Responsibilities
- Design, develop and optimize biochemical binding assays, with a focus on AlphaScreen/AlphaLISA proximity formats.
- Optimize assay conditions to deliver robust signal windows.
- Support cell-based assays, including mammalian cell culture, plating and compound treatment, cell density and workflow optimization, acquire and analyze images on high-content imaging systems.
- Miniaturize and transfer assays to 384- and 1536-well formats; validate them for screening.
- Execute medium- to high-throughput screening campaigns and dose-response profiling to support hit identification and SAR.
- Identify and triage assay interference using counter-screens and orthogonal formats.
- Manage compound and sample workflows, including plate preparation and retrieval from automated sample storage systems.
- Analyze, QC and report data in Scilligence; maintain complete, contemporaneous ELN records.
- Write and maintain SOPs and assay reports; present results and issues clearly at project team meetings.
- Qualify critical reagents in partnership with protein sciences, and track lot-to-lot performance.
Requirements
- BS in Biochemistry, Biology, Chemistry or a related field with 7+ years of relevant biopharmaceutical industry experience, or an MS with 5+ years.
- Direct, hands-on experience developing and running AlphaScreen and/or AlphaLISA assays, including troubleshooting signal window, hook effect and compound interference.
- Demonstrated track record of developing and validating biochemical binding assays for medium- to high-throughput screening in 384- and/or 1536-well formats.
- Proficiency with liquid handlers and plate-based workflows and multimode plate readers capable of Alpha detection.
- Familiarity with high-content imaging equipment.
- Sound understanding of binding kinetics and pharmacology.
- Strong data analysis skills, including curve fitting and assay statistics.
- Excellent documentation practices in an electronic lab notebook.
- Experience with cell culture and cell-based assays supporting drug screening.
- Strong attention to detail and scientific rigor.
- Proactive, transparent communicator who works independently day to day while collaborating closely with chemistry and biology teams.
- Experience operating and maintaining automated compound/sample storage systems, including inventory management, cherry-picking and plate reformatting.
- Experience with orthogonal binding and proximity formats for hit confirmation.
- Experience with integrated robotic screening systems and scheduling software.
- Experience screening against integrins, other cell-surface receptors or protein–protein interaction targets.
- Scripting or automation skills for data processing.
Skills
- AlphaScreen/AlphaLISA
- Biochemical binding assays
- Medium- to high-throughput screening
- Assay development
- Assay optimization
- Mammalian cell culture
- High-content imaging
- Data analysis
- Electronic lab notebook (ELN)
- SOP writing
- Reagent qualification
- Liquid handlers
- Plate-based workflows
- Multimode plate readers
- Binding kinetics
- Pharmacology
- GraphPad Prism
- Genedata Screener
- Automated compound/sample storage systems
- Orthogonal binding and proximity formats
- HTRF/TR-FRET
- Fluorescence polarization
- SPR
- BLI
- Integrated robotic screening systems
- Scripting
- Automation
- Python
- R
- KNIME
Location
- South San Francisco
Work Type
- Full-time
Experience Level
- 7+ years of relevant biopharmaceutical industry experience (BS)
- 5+ years of relevant biopharmaceutical industry experience (MS)
Education Level
- BS in Biochemistry, Biology, Chemistry or a related field
- MS
Salary/Compensations
- $125K – $138K
Benefits
- Equity
- Annual target bonus
About the Company
- At Pliant, we believe differentiated science can make a meaningful impact.
- We see our success as a direct result of the central role that each team member plays in advancing our mission to develop therapies that target the disease at its source.
- We are seeking creative, curious and collaborative individuals who dare to succeed, are committed to making an important impact on patient’s lives and will help us build one Pliant!
Equal Opportunity
- To understand how Pliant complies with and manages your personal information with respect to the California Consumer Privacy Act, please visit https://pliantrx.com/ccpa-applicant-and-staff-privacy-notice/.