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About the Role
This role builds our cell lines from the start. You would engineer, validate and bank the human cell lines our functional genomics screens run on, in a lab where the methods you set become the standard.
Responsibilities
- Generate CRISPR-edited lines, including knockouts, knock-ins and endogenous tags, isogenic disease and corrected pairs, and inducible systems.
- Isolate clones and validate them by genotyping, sequencing, karyotype and pluripotency QC, and off-target checks.
- Differentiate iPSCs into neurons and other lineages and characterise them by immunostaining and imaging.
- Build and manage the cell banks, with records good enough that anyone can trace a vial back through every passage, edit and QC result.
- Make protocols more consistent and move them into plate-based formats ready for automation.
- Run core iPSC and cell culture workflows independently and to a consistent standard.
- Learn the cell-line register, the QC gates a line passes through, and how its history is recorded.
- Take a first engineered line from guide design through editing and into clonal isolation.
- Write or revise at least one cell maintenance protocol, including how failures are captured.
- Bring that first line through validation, with banking under way.
- Improve one protocol for consistency or move it into a plate-based format.
Requirements
- A PhD in cell biology, stem cell biology, neuroscience or a related field, or an MSc and two to three years of relevant lab experience.
- Strong hands-on experience culturing iPSCs or other demanding cell types, such as primary cells, neurons or organoids.
- Practical CRISPR genome editing and clonal line generation, with the molecular biology behind it (cloning, PCR, Sanger and NGS genotyping).
- Excellent aseptic technique and careful, traceable record-keeping.
Skills
- CRISPR genome editing
- Clonal line generation
- Molecular biology (cloning, PCR, Sanger and NGS genotyping)
- Aseptic technique
- Record-keeping
- iPSC culture
- Cell culture
- CRISPR-edited lines
- Genotyping
- Sequencing
- Karyotype
- Pluripotency QC
- Off-target checks
- Cell differentiation
- Immunostaining
- Imaging
- Cell bank management
- Protocol development
- Automation
- Forward programming of iPSCs into neurons or other cell types by transcription factor induction (e.g., NGN2)
- Lentiviral production and transduction
- Fluorescence or high-content imaging
- Flow cytometry for single-cell sorting
- Lab automation (e.g., liquid handlers or plate-based workflows)
Location
- King’s Cross, London
Work Type
- Lab-based
- Full-time
Experience Level
- MSc and two to three years of relevant lab experience
Education Level
- PhD in cell biology, stem cell biology, neuroscience or a related field
- MSc
Benefits
- 30 days of annual leave plus public holidays
- Pension with a 10% employer contribution
- Top-tier Bupa private health cover
About the Company
- AI for biology has a data problem. The data that matters most doesn’t exist yet, so we’re building the infrastructure to produce it, with quality and traceability built in from day one.
- We’re in stealth and heads-down on execution. We’ll share more about what we’re building once you’ve spoken with the team.
Equal Opportunity
- We are an equal opportunity employer. We make hiring decisions on merit, scope-fit, and the strength of the working relationship we expect to build with each hire. Applications welcome from candidates of any background.