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About the Role
The laboratory of Benoit Bruneau at Gladstone Institutes is seeking a Research Associate to study gene regulation during heart development using mouse models and stem cells. The role offers potential for publications and is ideal for candidates planning graduate or medical studies.
Responsibilities
- Maintain and expand induced pluripotent stem cell (iPSC) lines, including routine passaging, freezing, and thawing.
- Utilize differentiation protocols to generate cardiomyocytes and assess differentiation efficiency via flow cytometry.
- Perform molecular biology techniques such as PCR, RT-qPCR, and western blotting to analyze gene expression and protein levels.
- Conduct sequencing techniques like RNA-seq, ChIP-seq, and ATAC-seq to investigate gene regulation and epigenetic changes.
- Analyze experimental and sequencing data, and interpret results.
- Update records and log books, and assist in maintaining lab records and inventory.
- Assist with lab management functions, including ordering reagents and organizing inventory.
Requirements
- Associate's degree and 2+ years of relevant experience.
- Excellent verbal and written communication skills.
- Strong work ethic.
- Strong organizational skills with attention to detail and the ability to multitask.
- Excellent communication and interpersonal skills, with the ability to work effectively in a team setting.
Skills
- iPSC culture
- Cell culture
- Flow cytometry
- PCR
- Western blotting
- RT-qPCR
- Lab management (ordering, restocking, database maintenance)
- R or python programming (or equivalent)
- Next-generation sequencing, e.g., RNA-seq, ChIP-seq
- CRISPR/Cas9 genome editing
- Proficiency in Excel, Word, ImageJ Fiji, Photoshop and Illustrator
Location
- San Francisco
Work Type
- Full-time
Experience Level
- 2+ years of relevant experience
- At least 6 months practical experience in a research laboratory (preferred)
Education Level
- Associate's degree
- BA/BS in Biological sciences (preferred)
Salary/Compensations
- $60,147 - $69,307
Benefits
- Generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.
About the Company
- Gladstone Institutes is a world-renowned independent, nonprofit biomedical research organization dedicated to solving challenging diseases.
- Scientists are leaders in neuroscience, cardiovascular disease, immunology, stem cell biology, and emerging areas of biomedical innovation.
- Fosters a collaborative, inclusive culture where employees are encouraged to grow professionally.
- Approximately 600 employees.
Equal Opportunity
- Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic. We make all employment decisions so as to further this principle of equal employment.