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About the Role
We are hiring a hands-on Senior Research Associate to build and run the cell-based assays and engineered cell lines behind our drug discovery programs. You will work day to day with a senior scientist in R&D, owning bench execution while helping shape assay strategy.
Responsibilities
- Maintain mammalian cell lines, engineer them with RNP-based CRISPR (knockouts and knock-ins), generate validated stable lines, and turn them into robust functional assays.
- Culture, expand, and maintain mammalian cell lines under strict aseptic technique; monitor health, viability, density, and passage history.
- Maintain cryopreserved stocks, run mycoplasma and identity checks, and keep accurate lineage records.
- Optimize RNP-based CRISPR/Cas editing in mammalian cell lines: single and double knockouts.
- Design and execute targeted knock-in strategies, including donor design, delivery optimization, and editing-efficiency assessment.
- Genotype and validate edits by sequencing, qPCR or digital PCR, and protein-level readouts.
- Perform transient transfections to test constructs quickly, optimizing reagent, nucleic acid amount, and harvest timing, and confirming expression before committing to a stable line.
- Generate stable lines using viral and non-viral delivery; perform clonal selection, expansion, functional validation, and banking.
- Characterize clones for expression level, assay performance, and stability over passage.
- Develop, optimize, and run functional assays: plate-based, reporter and proximity-based assays; flow cytometry; and other cellular functional readouts.
- Optimize seeding density, stimulation conditions, dose range, incubation time, controls, and acceptance criteria to improve sensitivity, dynamic range, and reproducibility.
- Prepare test articles, reagents, controls, and assay plates according to established protocols and study plans.
- Analyze dose-response data, fit curves, assess assay quality (Z', signal window, variability), and flag trends, outliers, and deviations.
- Keep complete, timely records in the electronic lab notebook, consistent with data-integrity expectations.
- Present results at team meetings and contribute figures and data to technical reports.
- Troubleshoot routine assay and culture problems, escalate significant deviations, and partner with colleagues to improve method robustness and transferability.
- Support reagent preparation, inventory, equipment upkeep, sample tracking, and safety compliance.
- Manage assigned work to agreed timelines.
Requirements
- Applicants must be authorized to work for any employer in the U.S. We are unable to sponsor or take over sponsorship of an employment Visa at this time.
- Bachelor's or Master's degree in biology, cell biology, bioengineering, biochemistry, immunology, or a related discipline, or an equivalent combination of education and experience.
- Relevant hands-on laboratory experience.
- Demonstrated mammalian cell culture experience with strong aseptic technique, including routine maintenance of multiple lines in parallel.
- Hands-on experience with CRISPR gene editing: knockout generation, and ideally knock-in, with validation of edited clones.
- Experience with transient transfection, and with generating and validating stable cell lines, including clonal selection and banking.
- Experience executing at least one cell-based assay readout, such as flow cytometry, plate-based viability, reporter, or proliferation assays.
- Ability to analyze data, assess assay quality, communicate findings clearly, and work in a collaborative, multidisciplinary setting.
- Commitment to laboratory safety, research integrity, accurate documentation, and responsible handling of biological materials.
Skills
- Mammalian cell culture
- CRISPR gene editing
- CRISPR knockout
- CRISPR knock-in
- Stable cell line generation
- Cell-based assay development
- Flow cytometry
- Plate-based viability assays
- Reporter assays
- Proliferation assays
- Data analysis
- Assay quality assessment
- Communication
- Collaboration
- Laboratory safety
- Research integrity
- Accurate documentation
- Responsible handling of biological materials
- RNP-based CRISPR knockout
- RNP-based CRISPR knock-in
- Lonza Nucleofector platforms
- High-throughput assay platforms
- Automated assay platforms
- 96-channel electronic pipetting systems
- 384-channel electronic pipetting systems
- Benchtop pipetting robots
- BRET
- TR-FRET/HTRF
- Proximity-based assay formats
- Molecular biology
- Construct design
- Primer design
- Cloning
- qPCR
- Digital PCR
- Western blot
- Drug discovery
- Assay qualification
- Design of experiments
- Dose-response analysis
- Curve fitting
- Acceptance criteria
- Statistical assessment of assay variability
- Neuronal cell models
- CNS-relevant cell models
- Multiplexed editing
- Triple knockouts
- Combined knockout and knock-in lines
Location
- South San Francisco
Work Type
- Onsite
- Laboratory-based
Experience Level
- Senior
Education Level
- Bachelor's degree in biology, cell biology, bioengineering, biochemistry, immunology, or a related discipline
- Master's degree in biology, cell biology, bioengineering, biochemistry, immunology, or a related discipline
- Equivalent combination of education and experience
Benefits
- Competitive compensation package with meaningful equity.
- Comprehensive health, dental, and vision coverage.
- Annual team gatherings and company events.
- Free lunch, snacks, beverages, and onsite gym access (for in-office employees).
About the Company
- Deep Origin is building ambitious science in a fast-moving environment, combining AI and physics-based methods with wet-lab biology to advance drug discovery.
- The people who thrive here are accountable, adaptable, rigorous, humble, and direct.
- Ownership through to the result.
- Judgment under incomplete information.
- Exacting about the work, unguarded about yourself.
- Transparency as a working habit.