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About the Role
The Molecular Biologist II will provide molecular biology and cell culture support for an ion channel drug discovery group focused on pain research. This individual will generate DNA constructs for wild-type and mutant ion channels, support cell line development for electrophysiology and biochemical assays, and contribute to the development of fluorescence-based pharmacology assays. The role requires solid hands-on skills in standard molecular biology and mammalian cell culture, strong attention to detail, and a good work ethic.
Responsibilities
- Design and generate DNA constructs encoding wild-type and mutant ion channels, including site-directed mutagenesis, to support structure-function studies and binding site mapping.
- Perform standard molecular cloning techniques, including PCR, restriction digestion, ligation, and plasmid preparation.
- Verify constructs via restriction digest, gel electrophoresis, and sequence analysis; troubleshoot cloning issues independently.
- Culture and maintain mammalian cell lines; perform transient and stable transfections for ion channel expression.
- Generate and characterize stable cell lines for use in electrophysiology, binding, and fluorescence-based assays.
- Support development and optimization of fluorescence-based assays (e.g., FLIPR, dye-based ion flux assays) for ion channel pharmacology.
- Perform DNA/RNA expression analysis (e.g., qPCR, gel-based methods) to confirm construct expression and cell line fidelity.
- Maintain accurate electronic laboratory notebook (eLN) records and clear documentation of protocols and results.
- Collaborate closely with electrophysiologists and pharmacologists to align construct and cell line design with experimental needs.
- Accurately report and record test data in electronic notebook or handwritten notebooks as needed in a timely manner.
- Prepare a variety of equipment for testing using prescribed standards / specifications.
- Maintain supplies, lab cleanliness and analytical instrumentation, as required.
- Prepare stock solutions, and keeping shelves and reagent stocked.
- Maintain up to date knowledge of current methods and products to be tested.
- Follow the guidelines set forth with clients and in the Bureau Veritas Consumer Products Services, Inc Quality, Health, Safety, Security and Environmental policies and procedures.
- Comply with clients and Bureau Veritas Consumer Products Services, Inc management systems in accordance with appropriate regulatory agencies.
- Adhere to the requirements of the BV-CPS Quality System.
- Other duties as defined by Manager, department needs and workload.
Requirements
- Solid hands-on experience with mammalian cell culture, including transient and stable transfection.
- Proficiency in standard molecular biology techniques: cloning, PCR, restriction digestion, gel electrophoresis, and plasmid preparation.
- Experience with site-directed mutagenesis and DNA sequence analysis is a plus.
- Familiarity with fluorescence-based assay formats (e.g., FLIPR, dye-based assays) is a plus.
- Written and verbal communication skills including the ability to communicate effectively in a group setting, with colleagues and clients.
- Organizational and time management skills.
- Ability to perform multiple tasks.
- Ability to organize and prioritize workload to meet or exceed deadlines.
- Individual and Team work ethic.
- Proficiency with Windows, Word & Excel.
- Attention to detail.
- Positive attitude, self-motivated, high level of engagement.
- Champions company values including team engagement.
- Ability to present both facts and recommendations effectively.
- Technical problem solving and process improvement skills.
Skills
- Mammalian cell culture
- Transient transfection
- Stable transfection
- Molecular cloning
- PCR
- Restriction digestion
- Gel electrophoresis
- Plasmid preparation
- Site-directed mutagenesis
- DNA sequence analysis
- Fluorescence-based assay formats
- FLIPR
- Dye-based assays
- Written communication
- Verbal communication
- Organizational skills
- Time management
- Multitasking
- Teamwork
- Windows
- Word
- Excel
- Attention to detail
- Problem solving
- Process improvement
Location
- South San Francisco, CA
Work Type
- Full-time
Experience Level
- Minimum of two (2) years of experience with relevant laboratory experience with molecular biology and mammalian cell culture techniques.
Education Level
- Bachelor’s or Master’s degree in Molecular Biology, Cell Biology, Biochemistry, or closely related field
- An equivalent combination of education and experience may be accepted in lieu of above.
Salary/Compensations
- $34.00-$40.00 per hour
About the Company
- Bureau Veritas Consumer Products Services, Inc