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Responsibilities
- Conduct in vitro screening and validation of novel therapeutic agents in AML cell lines and primary patient samples.
- Perform and analyze flow cytometry experiments, including immunophenotyping for leukemia engraftment and apoptosis/cell cycle assays.
- Execute molecular biology techniques, including Western blotting, RNA isolation and RT-qPCR.
- Maintain mammalian cell cultures and perform standard cell-based assays.
- Assist with in vivo studies using mouse models of leukemia, including oral gavage, IP and SC injections, and mice colony maintenance.
- Maintain accurate experimental records and analyze data.
- Prepare buffers and stock materials and reagents.
- Perform a moderate number of simple or moderate technical procedures.
- Occasionally be responsible for ordering routine supplies.
- Occasionally analyze data.
Requirements
- Require occasional guidance.
- Rarely modify technical procedures.
- Rarely train other lab members.
- Rarely guide other lab members.
- A simple / limited understanding of scientific literature and the research goals and rationale is required.
- Rarely interpret data.
- Rarely make written contributions to papers and grant proposals.
Skills
- Flow cytometry
- Immunophenotyping
- Apoptosis/cell cycle assays
- Western blotting
- RNA isolation
- RT-qPCR
- Mammalian cell culture
- Cell-based assays
- Mouse models of leukemia
- Oral gavage
- Intraperitoneal (IP) injections
- Subcutaneous (SC) injections
- Mice colony maintenance
- Data analysis
- Scientific literature interpretation
Experience Level
- Zero to two years of related experience
Education Level
- Bachelor’s Degree in a related science or equivalent skills and related experience
Salary/Compensations
- USD $28.72/Hr. - USD $28.72/Hr.