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Responsibilities
- Execute recombinant protein purification using ÄKTA systems and gravity column chromatography.
- Run purification workflows from small-scale runs to large-scale production to ensure consistent yield and purity.
- Independently troubleshoot routine expression and purification protocols to improve process efficiency.
- Perform protein quantification, quality assessment, and characterization to ensure deliverables meet project specifications.
- Maintain detailed inventory records of purified proteins and raw materials.
- Strictly adhere to Standard Operating Procedures (SOPs) and documentation standards for data integrity.
- Maintain clear written and verbal communication with your supervisor and cross-functional teams.
- Present scientific findings at team meetings.
Requirements
- Proven experience with a wide range of protein purification techniques.
- Experience working with various chromatography resins and instrumentation (e.g., ÄKTA systems).
- Strong attention to detail.
- Ability to work independently in a fast-paced environment.
- Proactive team-player mindset.
- Familiarity with laboratory safety protocols.
- Experience maintaining rigorous scientific documentation.
- Prior experience transitioning from laboratory-scale to pilot-level manufacturing is preferred.
- Proficiency in column packing procedures is preferred.
- Must be able to move about the laboratory and office environment regularly.
- Must have fine motor skills and steady hands to handle delicate instruments, operate laboratory equipment, pipette accurately, and perform repetitive benchwork with precision.
- Must be able to lift and carry up to 40 pounds occasionally.
- Must have sufficient visual acuity to read small print on labels, instrument displays, and computer monitors.
- Close vision, color vision, and the ability to adjust focus are required.
- Frequently exposed to typical laboratory conditions, including biological samples, chemicals, and reagents.
- Must be able to wear appropriate personal protective equipment (PPE) such as lab coats, gloves, and safety goggles as required.
Skills
- ÄKTA systems
- Gravity column chromatography
- Protein quantification
- Protein quality assessment
- Protein characterization
- Inventory management
- Standard Operating Procedures (SOPs)
- Scientific documentation
- Written communication
- Verbal communication
- Teamwork
- Laboratory safety protocols
- Column packing
Location
- South San Francisco
Work Type
- Onsite
- Laboratory
- Office
Experience Level
- General guidance of a supervisor
- Independently troubleshoot routine expression and purification protocols
- Consulting with a supervisor on complex issues or major process changes
- Prior experience transitioning from laboratory-scale to pilot-level manufacturing is preferred
Education Level
- Bachelor’s degree in Chemical Engineering, Biochemistry, Biotechnology or a related scientific discipline.