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About the Role
Researchers in the Biology Department at Brookhaven National Laboratory seek to unravel and fully describe the complexities of biological systems, focusing on molecular and cellular mechanisms underlying biomolecular metal uptake, transport, and homeostasis. The goal is to translate these insights into protein engineering strategies for selective metal binding, enrichment, and recovery, leveraging the unique capabilities of Brookhaven’s National Synchrotron Light Source-II and Laboratory for Biomolecular Structure. The Biological Associate will work closely with the Principal Investigator and/or other research staff to advance research projects on increasing basic understanding and engineering of microbes and plants for metal sequestration, utilization, and biocatalysis.
Responsibilities
- Perform protein expression and purification using both eukaryotic and prokaryotic expression systems.
- Conduct comprehensive biophysical and biochemical characterization of proteins, including enzyme activity assays and protein-ligand binding/affinity measurements.
- Collaborate with AI and computational teams to design, engineer, and screen protein targets.
- Apply cryo-electron microscopy (cryo-EM) and/or X-ray crystallography for high-resolution structure determination of proteins and macromolecular complexes.
- Execute end-to-end cryo-EM workflows, including sample and grid preparation, data collection, image processing, 3D reconstruction, and atomic model building and refinement.
- Work effectively with research staff across different disciplines and levels of expertise in a multidisciplinary, collaborative research environment.
- Communicate scientific findings clearly and effectively through strong interpersonal, written, verbal, and presentation skills.
- Prepare, analyze, and present experimental data for scientific publications, presentations, and internal research reports.
- Maintain a safe, secure, organized, and compliant laboratory environment in accordance with institutional and regulatory requirements.
Requirements
- Bachelor’s degree in plant biology, biochemistry, or a related field.
- Minimum of three years of related work experience, including biological laboratory research.
- Demonstrated hands-on experience conducting biochemistry, molecular biology, or biotechnology research involving plants or microbes.
- Experience in diverse molecular biology and biochemistry techniques, including DNA/RNA extractions, PCR, gel electrophoresis, restriction enzyme digestion and ligation, protein expression and purification using both eukaryotic and prokaryotic expression systems.
- Experience in structure determination of metal transporters and metal-binding proteins.
- Experience in operating analytical instruments such as FPLC and performing data analyses.
- Ability to multi-task and meet deadlines.
- Ability to work independently with a strong work ethic and self-motivation.
- Clear and concise oral and written communication and organizational skills.
- Demonstrated laboratory coordination.
- Ability to plan and execute experiments/schedules effectively.
- Ability to track performance and report activities as required.
Skills
- Protein expression and purification
- Eukaryotic and prokaryotic expression systems
- Biophysical and biochemical characterization of proteins
- Enzyme activity assays
- Protein-ligand binding/affinity measurements
- AI and computational collaboration
- Cryo-electron microscopy (cryo-EM)
- X-ray crystallography
- Sample and grid preparation
- Data collection
- Image processing
- 3D reconstruction
- Atomic model building and refinement
- Interpersonal skills
- Written communication
- Verbal communication
- Presentation skills
- Scientific data analysis
- Laboratory safety and compliance
- Molecular biology techniques
- Biochemistry techniques
- Biotechnology research
- DNA/RNA extractions
- PCR
- Gel electrophoresis
- Restriction enzyme digestion and ligation
- Structure determination
- Metal transporters
- Metal-binding proteins
- FPLC operation
- Data analysis
- Multi-tasking
- Time management
- Independent work
- Work ethic
- Self-motivation
- Organizational skills
- Laboratory coordination
- Experimental planning
- Schedule execution
- Performance tracking
- Activity reporting
- Advanced molecular cloning
- Synthetic biology approaches
- Transient gene expression in tobacco
- Membrane protein purification
- HEK293 cell growth maintenance
- Cell-based assays
Location
- Upton, NY
Work Type
- Onsite
- 1 year term position with the possibility of renewal
Experience Level
- Minimum of three years of progressively responsible related work experience
Education Level
- Bachelor’s degree in plant biology, biochemistry, or a related field
- PhD’s degree in plant biology, biochemistry, or relevant field strongly preferred
Salary/Compensations
- $80,600 - $112,833 / year
Benefits
- Comprehensive employee benefits program
About the Company
- Brookhaven National Laboratory delivers discovery science and transformative technology to power and secure the nation’s future.
- Brookhaven Lab is a multidisciplinary laboratory with seven Nobel Prize-winning discoveries, 37 R&D 100 Awards, and more than 70 years of pioneering research.
- The Lab is primarily supported by the U.S. Department of Energy’s (DOE) Office of Science.
- Brookhaven Science Associates (BSA) operates and manages the Laboratory for DOE.
- BSA is a partnership between Battelle and The Research Foundation for the State University of New York on behalf of Stony Brook University.
- BSA salutes our veterans and active military members with careers that leverage the skills and unique experience they gained while serving our country.
Equal Opportunity
- Guided by our core values of integrity, responsibility, innovation, respect, and teamwork, Brookhaven Science Associates is an Equal Employment Opportunity Employer-Vets/Disabled.
- We are committed to fostering a respectful and collaborative environment that fuels scientific discovery.
- We consider all qualified applicants without regard to any characteristic protected by law.
- All qualified individuals are encouraged to apply.
- We ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.
- BSA employees are subject to restrictions related to participation in Foreign Government Talent Recruitment Programs, as defined and detailed in United States Department of Energy Order 486.1A.