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About the Role
We are seeking a motivated Postdoctoral Researcher I to join Dr. Fei-Fei Liu’s laboratory at the Princess Margaret Cancer Research Tower, University Health Network. They will investigate the cellular and molecular mechanisms of radiation-induced fibrosis and contribute to identifying and evaluating potential therapeutic strategies. This position combines bioinformatics with experimental cell and molecular biology. The successful candidate will analyse biological datasets, develop research hypotheses, and test these hypotheses through laboratory experiments. We are looking for someone who takes initiative, approaches research critically, and works effectively within a collaborative team.
Responsibilities
- Design and conduct studies of the cellular and molecular mechanisms of radiation-induced fibrosis in collaboration with the Principal Investigator.
- Analyse gene-expression and other molecular datasets using bioinformatics and appropriate statistical methods to identify relevant pathways and candidate therapeutic targets.
- Integrate computational findings with cell-based experiments to test mechanisms and validate candidate targets.
- Maintain mammalian cell cultures, including primary cells, and perform cell and molecular biology assays such as RT-qPCR, Western blotting, ELISA, and microscopy.
- Evaluate candidate anti-fibrotic compounds and optimise experimental assays and protocols.
- Maintain accurate experimental records and documented analysis workflows, with appropriate quality control to support reproducible research.
- Prepare manuscripts, figures, reports, and presentations, and contribute to grant and fellowship applications.
- Collaborate with laboratory members and external partners, contribute to mentoring trainees, and follow applicable laboratory safety and research ethics requirements.
Requirements
- PhD in cell biology, molecular biology, biochemistry, bioinformatics, radiation biology, or a related discipline, completed by the start of the appointment.
- Experience in bioinformatics, including the analysis and interpretation of biological datasets, with proficiency in R or Python and relevant statistical and data visualisation methods.
- Hands-on experience with mammalian cell culture, aseptic technique, and cell-based assays.
- Experience with molecular biology and biochemical techniques, such as RNA or DNA extraction, RT-qPCR, Western blotting, and ELISA.
- Ability to design experiments, critically evaluate results, troubleshoot experimental and analytical workflows, and advance a research project independently.
- Strong written and verbal communication skills, including scientific writing and presentation of research findings.
- Strong organisational and record-keeping skills, attention to detail, and the ability to work collaboratively and follow laboratory safety practices.
- Experience with small-animal models, animal handling, and tissue collection.
- Research experience in fibrosis, radiation responses, wound healing, or tissue repair, preferred.
- Experience analysing bulk or single-cell RNA-sequencing data and performing pathway or gene-set enrichment analyses, preferred.
- Experience isolating primary cells from human or mouse tissues, or performing immunofluorescence and quantitative microscopy, preferred.
- Experience with small-animal models, animal handling, and tissue collection, preferred.
Skills
- Bioinformatics
- R
- Python
- Statistical methods
- Data visualisation
- Mammalian cell culture
- Aseptic technique
- Cell-based assays
- RNA extraction
- DNA extraction
- RT-qPCR
- Western blotting
- ELISA
- Microscopy
- Experimental design
- Critical evaluation
- Troubleshooting
- Scientific writing
- Presentation
- Organisation
- Record-keeping
- Attention to detail
- Collaboration
- Laboratory safety
- Small-animal models
- Animal handling
- Tissue collection
- Fibrosis research
- Radiation response research
- Wound healing research
- Tissue repair research
- RNA-sequencing data analysis
- Pathway analysis
- Gene-set enrichment analysis
- Primary cell isolation
- Immunofluorescence
- Quantitative microscopy
Location
- Allan Slaight Medical Innovation Labs
Work Type
- On site
- Day shifts
- Monday to Friday
- Occasional weekend work
- Temporary Full-time
- 1 year contract
Experience Level
- Postdoctoral Researcher I
Education Level
- PhD
Salary/Compensations
- $54,902 - $93,333 per year
Benefits
- Competitive offer packages
- Member of the Healthcare of Ontario Pension Plan (HOOPP)
- Close access to Transit
- UHN shuttle service
- Flexible work environment
- Opportunities for development and promotions
- Multiple corporate discounts (travel, restaurants, parking, phone plans, auto insurance)
- On-site gyms
About the Company
- UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital.
- With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre.
- As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care.
- UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine.
- UHN is a research hospital affiliated with the University of Toronto.
- UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.
Equal Opportunity
- UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace.
- Requests for accommodation can be made at any stage of the recruitment process.
- Applicants need to make their requirements known.