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About the Role

Our lab is a multidisciplinary research program focused on understanding chromatin architecture, epigenetic regulation, and tumor heterogeneity in cancer. We utilize advanced techniques like 3D genome mapping and next-generation sequencing to identify regulatory mechanisms for precision oncology and biomarker discovery. We are seeking a motivated Research Technician with strong wet-lab experience to support experimental projects in cancer epigenomics and 3D genome biology.

Responsibilities

Perform and optimize molecular biology and functional genomics experiments related to cancer epigenomics.
Carry out chromatin and sequencing-based assays, including Hi-C, HiChIP, ATAC-seq, CUT&RUN, CUT&Tag, and related methods.
Prepare next-generation sequencing libraries across multiple assay types.
Work with patient-derived cancer models, including organoids or related culture systems.
Support sample processing, quality control, library preparation, and coordination of sequencing submissions.
Maintain accurate experimental records, protocols, sample inventories, and laboratory documentation.
Assist with troubleshooting and optimization of complex wet-lab protocols.
Coordinate with computational scientists and other team members to support data generation, interpretation, and project progress.
Contribute to the generation of figures, summaries, and experimental documentation for manuscripts, grants, presentations, and reports.
Assist with ordering, reagent preparation, equipment maintenance, and general laboratory organization.
Support training of junior lab members or students in established protocols, as appropriate.
Contribute to a collaborative, rigorous, and well-organized lab environment.

Requirements

Minimum of 3 years of relevant wet-lab research experience required.
Strong hands-on experience with molecular biology and genomics techniques.
Experience with next-generation sequencing library preparation.
Experience with chromatin-based or epigenomics assays, such as ATAC-seq, CUT&RUN, CUT&Tag, Hi-C, HiChIP, Capture-C, or related methods.
Experience with patient-derived organoid culture or other cancer model systems.
Ability to follow, optimize, and troubleshoot complex experimental protocols.
Strong organizational skills and careful attention to detail.
Ability to maintain clear and accurate experimental records.
Excellent communication skills and fluency in English, written and spoken.
Ability to work effectively in a collaborative, multidisciplinary research environment.
Ability to manage multiple experimental tasks and timelines.
Direct experience with 3D genome assays, such as Hi-C, HiChIP, Capture-C, Micro-C, or related methods an asset.
Experience with single-cell or spatial genomics workflows an asset.
Experience with low-input or challenging clinical/research samples an asset.
Basic familiarity with genomic data analysis concepts and tools, including R, Python, command-line environments, or sequencing QC metrics an asset.
Experience working in a translational cancer research environment an asset.
Prior experience contributing to manuscripts, reports, or grant-related experimental summaries an asset.
Criminal Record Check may be required.

Skills

Molecular biology
Genomics
Epigenomics
Cancer biology
Chromatin conformation assays
Functional epigenomics
Next-generation sequencing library preparation
Patient-derived model systems
3D genome mapping
Chromatin profiling
Multi-omic approaches
Organoid culture
R
Python
Command-line environments
Sequencing QC metrics

Location

Princess Margaret Cancer Centre

Work Type

Temporary Full-Time
1 year contract

Experience Level

Minimum of 3 years of relevant wet-lab research experience

Education Level

Bachelor's degree in Molecular Biology, Cancer Biology, Genetics, Genomics, Biochemistry, Cell Biology, or a related field required
Master's degree an asset

Salary/Compensations

CAD 31.03 - CAD 46.84 hourly

Benefits

Competitive offer packages
Government organization
Member of the Healthcare of Ontario Pension Plan (HOOPP)
Close access to Transit and UHN shuttle service
A flexible work environment
Opportunities for development and promotions within a large organization
Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

About the Company

UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital.
With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre.
As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care.
UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine.
UHN is a research hospital affiliated with the University of Toronto.
UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.

Equal Opportunity

UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace.
Requests for accommodation can be made at any stage of the recruitment process.
Applicants need to make their requirements known.