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About the Role
Seeking a Senior Research Associate III (SRA) to support research on T cell development, thymic epithelial biology, and thymic regeneration. The SRA will lead in vivo rodent research projects focusing on thymic and T cell functions, requiring extensive knowledge in T cell and thymus biology, colony management, surgical procedures, and downstream analyses.
Responsibilities
- Independently manage large, complex immunodeficient mouse colonies including NSG-athymic mice and other NSG strains.
- Perform routine animal husbandry tasks including breeding, weaning, genotyping and tagging; monitor colony health; coordinate with vivarium staff regarding logistics.
- Plan and execute long-term, complex dosing procedures including HSC engraftment and immunization; plan and perform blood collection; conduct survival surgeries including renal capsule transplantations, and post-op animal care.
- Conduct terminal tissue collection under aseptic conditions, including thymus, spleen, bone marrow and grafted tissues.
- Work with BSL2+ materials in animals.
- Oversee animal record keeping, including colony records, surgery logs and controlled substance use logs.
- Prepare tissue sections, perform IHC, IF and ICC staining, and execute confocal imaging.
- Perform multi-color FACS, including surface and intracellular staining.
- Perform DNA/RNA extractions, PCR/qPCR, western blot, molecular cloning, and NGS library preps.
- Maintain primary and cell line cultures, perform transfections, transductions, and AAV and lentivirus preps.
- Optimize and develop new protocols to address novel scientific questions, using scientific innovation.
- Process, analyze and organize experimental data using specialized software.
- Maintain meticulous lab notebooks and digital records.
- Assist the PI in drafting/updating IACUC/IBC protocols, writing manuscripts and presentations, preparing NIH/CIRM documentations.
- Ensure lab compliance with safety and regulatory guidelines.
- Assist with lab organization including electronic and paper record keeping.
- Oversee the maintenance, inspection and repair of lab equipment.
- Provide training and guidance to junior lab members, students and trainees.
Requirements
- BA/BS with a degree in a related science, including two years of recent/directly related work experience, utilizing the techniques/methods required by the position and demonstrating technical expertise in these areas; and/or equivalent experience/training
- Background in immunology, with specific knowledge in hematopoiesis and T cell development
- Demonstrated proficiency in mouse husbandry, breeding schemes, and multiple administration routes
- Proven ability to perform rodent survival surgery and tissue collection under aseptic conditions
- Experience with a wide range of wet lab skills, including cell culture, FACS, microscopy, and molecular biology techniques
- Proficiency with specialized scientific software such as FlowJo, ImageJ/LAS X, and GraphPad Prism
- Excellent organizational skills, strong record-keeping, and ability to work independently as a technical specialist
- Specialized scientific background in thymus development and TEC/thymocyte crosstalks
- Demonstrated surgical techniques including bone marrow transplantation, renal capsule transplantation, and adoptive transfer models
- Extensive experience with advanced multi-color FACS including spectral flow cytometry
- Experience working with BSL2+ materials
- Prior experience managing vendor relations, ordering, and compliance documentation (IACUC, EH&S, BSL-2)
Skills
- T cell development
- thymic epithelial biology
- thymic regeneration
- in vivo rodent research
- T cell biology
- thymus biology
- mouse colony management
- surgical procedures
- tissue analysis
- cellular analysis
- molecular analysis
- cell culture
- FACS
- Western blot
- immunohistochemistry
- confocal microscopy
- molecular biology
- FlowJo
- ImageJ/LAS X
- GraphPad Prism
Experience Level
- Senior
Education Level
- BA/BS
About the Company
- Department of Pediatrics