Research Associate, Therapeutic Technologies at Addition Therapeutics | CA, US | Rezi

Research Associate, Therapeutic Technologies at Addition Therapeutics

Research Associate, Therapeutic Technologies

Addition Therapeutics · CA, US

5 days ago

Research Associate, Therapeutic Technologies

Addition Therapeutics · CA, US

6 days ago
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About the Role

We are seeking a versatile, hands-on Research Associate to join our Therapeutic Technologies team. This role is central to transforming designs into data by producing RNA reagents and conducting cell-based assays to evaluate their performance. You will work closely with cross-functional teams, ensuring the quality and reliability of experimental data that drives our programs forward.

Responsibilities

  • Produce research-grade RNA via in vitro transcription (IVT), including template preparation, transcription, capping, tailing, DNase treatment, and purification.
  • Perform RNA quality control, including concentration, purity, and integrity analysis using spectrophotometry, fluorometry, and electrophoretic/capillary methods.
  • Maintain mammalian cell lines and disease-relevant models, ensuring assay-ready cells are available.
  • Deliver PRINT RNA into cells using transfection, lipofection, electroporation, and nucleofection techniques.
  • Execute readout assays such as reporter assays, ELISA, qPCR/ddPCR, flow cytometry, and microscopy.
  • Analyze, plot, and document experimental results in an electronic lab notebook, identifying anomalies and contributing to troubleshooting.
  • Assist with lab operations, including reagent and consumable inventory, sample tracking, and instrument maintenance.

Requirements

  • Bachelor's or Master's degree in molecular biology, biochemistry, cell biology, biotechnology, or a related field.
  • Approximately 1-2 years of hands-on laboratory experience, with industry experience preferred.
  • Proficiency in mammalian cell culture, including sterile technique, maintenance of multiple cell lines, and transfection.
  • Hands-on molecular biology experience, including RNA handling and RNase-free technique.
  • Demonstrated reliability, organization, and attention to detail, with the ability to produce reproducible data and document it clearly.
  • Ability to manage multiple concurrent tasks in a fast-paced environment.
  • Strong communication skills to present results to a cross-functional team.

Skills

  • In vitro transcription (IVT)
  • RNA purification
  • RNA quality control
  • Spectrophotometry
  • Fluorometry
  • Electrophoretic/capillary methods
  • Mammalian cell culture
  • Sterile technique
  • Transfection
  • Lipofection
  • Electroporation
  • Nucleofection
  • Reporter assays
  • ELISA
  • qPCR
  • ddPCR
  • Flow cytometry
  • Microscopy
  • Electronic lab notebook
  • Reagent inventory
  • Consumable inventory
  • Sample tracking
  • Instrument upkeep
  • mRNA/RNA reagent production
  • mRNA/RNA reagent QC
  • Capping
  • Tailing
  • TapeStation
  • Bioanalyzer
  • Fragment analyzer
  • Western blot
  • Fluorescence microscopy
  • Gene therapy
  • mRNA therapeutics
  • RNA-based therapeutics
  • Data analysis
  • R
  • Python
  • GraphPad Prism

Location

  • South San Francisco

Work Type

  • Full-time
  • Onsite

Experience Level

  • 1-2 years

Education Level

  • Bachelor's degree
  • Master's degree

Salary/Compensations

  • $78,000–$98,000

Benefits

  • Bonus eligibility
  • Equity incentives

About the Company

  • Addition Therapeutics is a biotechnology company focused on developing the next generation of gene therapy using its proprietary PRINT™ platform.
  • The PRINT™ platform enables the development of RNA-only therapeutics for inserting genetic elements into specific safe harbor sites within the genome.
  • The company operates from state-of-the-art labs in South San Francisco and is advancing programs across rare and common diseases.

Equal Opportunity

  • Addition Therapeutics is an equal opportunity employer.
  • We value diverse perspectives and are committed to building an inclusive team.