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About the Role

The Postdoctoral Associate will assist the Principal Investigator Dr. Raymond Bergan in the Department of Medicine. The incumbent will conduct research and ensure that all experiments are appropriately conducted following the policies and procedures of Stony Brook University.

Responsibilities

• Conduct research experiments in the field of cancer cell biology and cell motility, including in vitro and in vivo (mouse model) studies of prostate cancer cell migration and its therapeutic modulation by selective chemical probes.
• Collect and analyze data, including periodical/literature search and utilizing specialized skills in related field, such as mass spectrometry-based proteomics, functional enrichment and network analysis, and structural bioinformatics, to analyze the collected data.
• Participate/assist in manuscript writing for publication in scientific journals and/or presentations.
• Assist in grant writing.
• Lab maintenance, including equipment maintenance and ordering of supplies as needed.
• Attend Scientific Conferences and Meetings.

Requirements

• Doctoral Degree (PhD) or foreign equivalent in Cancer Biology, Molecular and Cellular Biology, Biochemistry, or a closely related field.
• Research Laboratory skills that include, but not limited to PCR, generation of stable lentiviral cell lines, western blotting, immunofluorescence staining and confocal microscopy; cell proliferation and migration assays, including Boyden chamber and wound-healing/scratch assays and In Vivo mouse techniques, including handling and restraint, oral gavage and subcutaneous intraperitoneal and intravenous (tail-vein) injections.
• Research Lab experience in cancer cell biology and cell motility/migration in prostate cancer models.
• Hands-on experience in mammalian cell culture, molecular cloning (Gibson, In-Fusion, and restriction enzyme cloning), primer and construct design in SnapGene, site-directed mutagenesis, and molecular knockout.
• Experience in mass spectrometry-based proteomics, including data deposition and retrieval through the PRIDE (Proteomics Identifications) database.
• Experience in bioinformatic data analysis, including pathway and ontology enrichment (ORA and GSEA) using clusterProfiler, WebGestalt, g:Profiler, Enrichr, or QIAGEN Ingenuity Pathway Analysis (IPA).
• Experience in protein–protein interaction and functional network analysis using the STRING database and Cytoscape.
• Experience in structural contextualization by mapping peptides and PTM sites onto 3D protein structures from the Protein Data Bank (PDB), AlphaFold DB, or UCSF ChimeraX.
• Proficiency in statistical analysis and data visualization using GraphPad Prism.
• Experience with scientific writing, peer reviewed publications and conference presentations.

Skills

• PCR
• Generation of stable lentiviral cell lines
• Western blotting
• Immunofluorescence staining
• Confocal microscopy
• Cell proliferation assays
• Cell migration assays
• Boyden chamber assays
• Wound-healing/scratch assays
• In Vivo mouse techniques
• Handling and restraint of mice
• Oral gavage in mice
• Subcutaneous injections in mice
• Intraperitoneal injections in mice
• Intravenous (tail-vein) injections in mice
• Mammalian cell culture
• Molecular cloning (Gibson, In-Fusion, and restriction enzyme cloning)
• Primer design
• Construct design in SnapGene
• Site-directed mutagenesis
• Molecular knockout
• Mass spectrometry-based proteomics
• Data deposition and retrieval through PRIDE database
• Bioinformatic data analysis
• Pathway and ontology enrichment (ORA and GSEA)
• clusterProfiler
• WebGestalt
• g:Profiler
• Enrichr
• QIAGEN Ingenuity Pathway Analysis (IPA)
• Protein–protein interaction analysis
• Functional network analysis using STRING database
• Cytoscape
• Structural contextualization
• Mapping peptides and PTM sites onto 3D protein structures
• Protein Data Bank (PDB)
• AlphaFold DB
• UCSF ChimeraX
• Statistical analysis
• Data visualization using GraphPad Prism
• Scientific writing
• Peer reviewed publications
• Conference presentations

Location

• Stony Brook University

Work Type

• Full-time
• Exempt

Experience Level

• Postdoctoral

Education Level

• Doctoral Degree (PhD)

Benefits

• Total rewards

About the Company

• The Research Foundation of SUNY is a private educational corporation.
• SUNY Research Foundation: A Great Place to Work.

Equal Opportunity

• Stony Brook University is committed to excellence in diversity and the creation of an inclusive learning, and working environment. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion, sex, pregnancy, familial status, sexual orientation, gender identity or expression, age, disability, genetic information, veteran status and all other protected classes under federal or state laws.