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About the Role

Addition Therapeutics is seeking a highly skilled and motivated Associate Scientist/Scientist to join its HIV Molecular and Cellular Biology team. The successful candidate will play an essential hands-on role in advancing our HIV CAR-T program. This role offers significant learning and growth opportunities in the field of genomic medicines and provides a unique opportunity to explore the multifaceted applications of PRINT™ technology.

Responsibilities

Generation of replication competent HIV reporter virus followed by virus titration.
Evaluate different cell lines expressing CAR-T target for killing efficiency.
Infection of primary activated PBMC / T-cells with subsequent flow analysis.
Establish in vitro HIV challenge assays to quantify CAR-T mediated killing of PBMC.
Perform and interpret multiparameter flow cytometry analyses to characterize CAR-T cell expression, target binding, killing, and analyze immune populations.
Support molecular efforts such as lentivirus and CAR-T construct cloning, IVTs, etc.
Integrate in vitro efficacy, cellular, and molecular readouts to guide program decisions.
Troubleshoot experimental challenges and drive improvements in study design and execution.
Maintain detailed and organized study records; contribute to study reports and presentations.

Requirements

PhD in Biology, Pharmacology, Immunology, Cancer Biology, or related field, with 4-6 years of relevant research or industry experience.
Comfortable working with replication-competent HIV in an appropriate biosafety setting.
Experience with culturing primary human PBMCs and isolating T-cell populations.
Experience assessing in vitro tumor killing/efficacy and CAR-T expansion or persistence.
Proficiency in multiparameter flow cytometry and flow cytometry data analysis.
Experience with ddPCR, ELISA/MSD, or related molecular/protein assays is preferred.
Strong experimental, design, analytical, troubleshooting, organizational, and communication skills.
Meticulous attention to detail and strong documentation practices including use of electronic notebook for record keeping.
A collaborative team player who fosters a culture of accountability, scientific rigor, and excellence and thrives in a fast-paced, results-oriented startup environment.
A self-motivated, driven individual with a passion to learn and can-do attitude.
Bachelor’s or master’s degree in chemistry, Biochemistry or related field with 2-4 years of experience.

Skills

HIV-1 field experience
CAR-T cells experience
Multiparameter flow cytometry
Intracellular cytokine staining and analysis
Lentiviral vector production
Transduction of primary T cells
Long-term coculture assays
ddPCR
ELISA/MSD
Molecular/protein assays
Experimental design
Analytical skills
Troubleshooting
Organizational skills
Communication skills
Documentation practices
Electronic notebook usage

Location

South San Francisco, California

Work Type

Full-time

Experience Level

4-6 years of relevant research or industry experience (PhD)
2-4 years of experience (Bachelor's/Master's)

Education Level

PhD in Biology, Pharmacology, Immunology, Cancer Biology, or related field
Bachelor’s or master’s degree in chemistry, Biochemistry or related field

Salary/Compensations

$112,000-$132,000

About the Company

Addition Therapeutics is at the forefront of biotechnology innovation, advancing transformative therapies utilizing its proprietary PRINT™ (Precise RNA-mediated Insertion of Transgenes) platform.
PRINT™ redefines genetic medicine by enabling development of RNA-only therapeutics capable of inserting any genetic element into specific safe harbor sites within the genome.
Operating from state-of-the-art labs in South San Francisco, our team of experienced scientists and drug developers is leading scientific breakthroughs while cultivating a vibrant, dynamic culture committed to profoundly impacting patient lives.

Equal Opportunity

We are an Equal Opportunity employer and will consider all qualified applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, or disability status.