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About the Role

This role focuses on designing, planning, and executing experiments within a single-cell RNA sequencing pipeline, from sample preparation to NGS sequencing library preparation and quality control. The position involves hands-on laboratory work, data analysis, and collaboration within a dynamic start-up environment.

Responsibilities

• Design, plan, and execute experiments using single-cell RNA sequencing pipeline from sample preparation and capture to NGS sequencing library preparation and QC with high precision.
• Prepare single-cell and single-nuclei samples for next-generation sequencing (NGS) and other applications.
• Conduct fluorescence and/or magnetic-activated cell sorting experiments.
• Optimize and improve workflow, and streamline processes.
• Collect, organize, and manage sample inventory.
• Organize and maintain detailed experimental records in accordance with industry standards.
• Manage reagent inventory and equipment calibration/maintenance.
• Collaborate with multidisciplinary teams and colleagues.
• Analyze data, interpret and present results at team meetings.
• Support other Discovery projects involving human neuronal cell culture and cell-based assays.

Requirements

• BS or MS degree in Biology, Molecular/Cell Biology or related field.
• Experience with single-cell NGS sample prep workflow (cell capture using microfluidics, cDNA and library preparation, QC and sequencing).
• Experience with flow cytometry and fluorescence activated and/or magnetic cell sorting (FACS/MACS).
• Familiarity with DNA/RNA library QC techniques (Tapestation, Bioanalyzer, Qubit, etc.).
• Working knowledge of standard molecular biology techniques (PCR, qPCR, gel electrophoresis, DNA/RNA extraction).
• Strong organizational and analytical skills.
• Willingness to perform repetitive tasks with high accuracy and superb attention to detail.
• Willingness to learn and expand skill set and contribute to emerging needs in a highly dynamic start-up environment.
• Ability to multitask while working within fast-paced timelines.
• High integrity, excellent written, verbal and interpersonal communication skills.
• Strong team player and problem-solver, able to work on teams and independently.

Skills

• Single cell RNA sequencing
• NGS sample preparation
• Flow cytometry
• FACS/MACS
• DNA/RNA library QC techniques
• Tapestation
• Bioanalyzer
• Qubit
• PCR
• qPCR
• Gel electrophoresis
• DNA/RNA extraction
• Single-cell RNA sequencing data analysis
• Histochemical techniques
• Basic microscopy
• Sterile human stem cell culture
• Neuronal cell culture
• Stem cell biology
• Neurodevelopmental biology

Location

• South San Francisco, CA, 94080

Work Type

• Onsite

Experience Level

• Various experience levels considered
• 2+ years of relevant hands on laboratory experience in academia or industry (preferred)

Education Level

• BS or MS degree in Biology, Molecular/Cell Biology or related field

About the Company

• We are aware of active recruitment scams using the Neurona name, where individuals pose as our recruiters or post fake remote job openings and make fraudulent job offers online.
• Please note, that we will never make an offer of employment without conducting multiple rounds of face-to-face interviews using secure video-conferencing technology.
• Additionally, we will never ask candidates to cash checks or make any payment to be considered for a position.
• If you believe you have been a victim of a phishing scam, please visit the Cybersecurity & Infrastructure Security Agency (CISA) at https://www.cisa.gov/be-cyber-smart/report-incident to learn how to report it.
• This job description is not intended to be exhaustive and may evolve as business needs change.
• Duties, responsibilities, and activities may be modified or assigned at any time with or without notice.