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About the Role

The Cell Line Development (CLD) Innovation Group is seeking a Senior Scientist I to join a talented and collaborative team developing next-generation CHO cell line platforms for the production of recombinant antibodies and complex biologics. This is a hands-on, laboratory-focused role for a versatile molecular biologist who will independently drive expression-optimization projects across both the vector and the host cell — from experimental design through execution and data interpretation.

Responsibilities

Independently conceive ideas and designs to investigate CHO expression technical improvements across both vector and host cell.
Design and test vector constructs in recombinant stable CHO cell lines for increased mAb titer.
Employ a variety of hands-on laboratory techniques, including transfection, aseptic CHO cell culture, DNA gene copy number analysis, RNA expression analysis, mAb titer measurement, cell engineering to knock in/out target genes, and in vitro transcription.
Receive externally sourced vector libraries and rapidly execute the associated transfection, screening, and clone-characterization studies to generate decision-quality data.
Operate and interpret data from core CLD instrumentation, including Octet (BLI titer), ViCell, CloneSelect Imager, microfluidic clone-screening platforms such as the Beacon, flow cytometry, and ddPCR / RT-qPCR copy-number and expression assays.
Share responsibility for equipment maintenance.
Apply a rigorous theoretical and practical understanding of recombinant protein expression from the genome to the protein.
Author and co-author cell line development technical reports and present data to the group and to leadership.
Contribute scientific insight to project teams.
Collaborate closely with cell culture, analytical, purification, and bioinformatics colleagues to meet project timelines.
Adhere to corporate standards for code of conduct, safety, materials handling, and good documentation practices.

Requirements

BS with a minimum of 10 years, or MS with a minimum of 8 years of experience, or PhD with no minimum years’ experience, in molecular biology, cell biology, biochemistry, or a related discipline.
Demonstrated ability to read the literature, review external sources, and gather inputs to synthesize technically sound ideas and design proper experimental controls.
Demonstrated ability to independently analyze experimental data, draw appropriate conclusions, and communicate them to a wide audience.
Demonstrated proficiency with designing and implementing vector improvements to increase recombinant antibody expression levels in stable CHO cells.
Demonstrated theoretical command of mammalian protein expression.
Proficiency with Geneious Prime or similar software for designing and annotating DNA vectors, primers, and probes, and performing sequence alignments.
Proficiency with cell engineering to knock in / knock out target genes using CRISPR/Cas or other suitable tools.
Proficiency with most or all of the following techniques and instrumentation: transfection, aseptic mammalian cell culture, Octet, ViCell, CloneSelect Imager, and microfluidic clone-screening platforms such as the Beacon.
Demonstrated ability to learn, understand, and master new experimental techniques.
Demonstrated ability to multi-task and work within timelines.
Strong scientific communication skills — verbal, written, and data presentation.
Detailed daily digital lab notebook record-keeping proficiency.
A candidate who combines hands-on wet-lab depth with the ability to think alongside the team and then execute independently.
Hands-on, industry cell line development experience in a CMC / drug-substance environment.
Substantial experience developing CHO expression platform improvements in an industrial/CMC setting.
Thorough understanding of the genetic and epigenetic factors governing mAb expression in recombinant stable CHO cells.
Previous experience working in CMC (Chemistry, Manufacturing and Controls) to contribute to the generation of recombinant CHO cell lines that will be used in GMP manufacturing.
Previous experience performing cellular analyses using flow cytometry.
Experience across multiple expression-engineering strategies beyond DNA copy number and well-known promoters.
Working knowledge of next-generation sequencing (NGS) for genomic DNA and RNA expression analysis.
Ability to match the appropriate NGS assay to the scientific question.
Hands-on experience with wet-lab NGS library preparation and the associated data analysis and interpretation.
Programming and data-science skills (e.g., Python, R) for analysis and visualization of cell line development data.

Skills

Molecular biology
Cell biology
Biochemistry
Transfection
Aseptic CHO cell culture
DNA gene copy number analysis
RNA expression analysis
mAb titer measurement
Cell engineering
In vitro transcription
Octet (BLI titer)
ViCell
CloneSelect Imager
Microfluidic clone-screening platforms (e.g., Beacon)
Flow cytometry
ddPCR / RT-qPCR
Geneious Prime
CRISPR/Cas
Mammalian protein expression
Genomic and epigenetic regulation
Transcriptional and translational control
Protein secretion machinery
mRNA stability
Next-generation sequencing (NGS)
Python
R

Location

US & Puerto Rico

Work Type

Full-time

Experience Level

Senior Scientist I
10 years experience (BS)
8 years experience (MS)
PhD (no minimum experience)

Education Level

Salary/Compensations

USD 96500 - USD 183500

Benefits

Paid time off (vacation, holidays, sick)
Medical/dental/vision insurance
401(k)
Short-term incentive programs

About the Company

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow.
We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio.
The Biologics CMC Development organization at AbbVie has a long and rich history of biologics development, leveraging comprehensive end-to-end CMC capabilities to advance complex biologic therapies, such as bi-specifics and novel modalities, from discovery through human clinical studies and eventual licensure.

Equal Opportunity

AbbVie is an equal opportunity employer and is committed to operating with integrity, driving innovation, transforming lives and serving our community.
Equal Opportunity Employer/Veterans/Disabled.
US & Puerto Rico only - to learn more, visit https://www.abbvie.com/join-us/equal-employment-opportunity-employer.html
US & Puerto Rico applicants seeking a reasonable accommodation, click here to learn more: https://www.abbvie.com/join-us/reasonable-accommodations.html